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nfo [2020-04-29 18:29:18]
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nfo [2020-04-29 18:29:18]

type IV apurinic/apyrimidinic endonuclease
locus
BSU_25130
pI
5.37
mw
32.92 kDa
protein length
297 aa Sequence Blast
gene length
894 bp Sequence Blast
function
repair of oxidative DNA damage in spores
product
type IV apurinic/apyrimidinic endonuclease
essential
no
synonyms
yqfS
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.2|A/P endonucleases]

    • Gene

    Coordinates
    2,593,281 2,594,174

    Phenotypes of a mutant

  • an ''[gene|FCDE5EA6E50A8C72A499E2F1FFB808DC86466A29|exoR] [gene|3C8BA04BA2BD0FEA28E99DA39BEB710CE3F5922D|nfo]'' double mutant is impaired in germination and spore outgrowth due to the accumulation of DNA lesions, this can be rescued by inactivation of ''[gene|2B091CCEE9E34D659771E39B1FC9050A145048AB|disA]'' [Pubmed|24244006]
  • an ''[gene|FCDE5EA6E50A8C72A499E2F1FFB808DC86466A29|exoR] [gene|3C8BA04BA2BD0FEA28E99DA39BEB710CE3F5922D|nfo]'' double mutant is sensitive to radiation [Pubmed|24123749]
  • sensitive to blue light-induced DNA damage [pubmed|30054368]
  • reduced resistance towards electron beams [pubmed|31948638]

    • The protein

    Catalyzed reaction/ biological activity

  • Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products (according to UniProt)

    • Protein family

  • AP endonuclease 2 family (single member, according to UniProt)

    • Structure

  • [PDB|3AAL] (from Geobacillus kaustophilus, 80% identity) [pubmed|21358045]

    • Expression and Regulation

    Operons

    genes
    [gene|C83BD80DE0FD187D07B914F64696438ED5130107|cshB]-[gene|3C8BA04BA2BD0FEA28E99DA39BEB710CE3F5922D|nfo]
    description
    [pubmed|22383849]
    view in new tab

    Biological materials

    Mutant

  • MGNA-C462 (yqfS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2460 NBRP B. subtilis, Japan]
  • available in [SW|Mario Pedraza-Reyes]'s lab
  • GP899 (Δ''nfo''::''kan'') and GP1502 (''nfo''::''cat''), available in [SW|Jörg Stülke]'s lab [pubmed|22178973]
  • BKE25130 (Δ[gene|3C8BA04BA2BD0FEA28E99DA39BEB710CE3F5922D|nfo]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GCCTATTCTCAGCAACAGGT, downstream forward: _UP4_TAAAAAATGGGGGATAACAC
  • BKK25130 (Δ[gene|3C8BA04BA2BD0FEA28E99DA39BEB710CE3F5922D|nfo]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GCCTATTCTCAGCAACAGGT, downstream forward: _UP4_TAAAAAATGGGGGATAACAC

    • References

    Reviews

  • 22933559

    • Original publications

  • 24244006,18203828,16237020,12949090,19930460,12486072,21441501,24123749,24123749,24914186,21358045,30054368,31948638